Journal: Cells

Article Title: Optimized Isolation and Characterization of C57BL/6 Mouse Hepatic Stellate Cells

doi: 10.3390/cells11091379

Figure Lengend Snippet: ( A ) After Nycodenz separation, high-lipid-content cells float (white ring) between the Nycodenz solution and the GBSS + NaCl solution (yellow arrow). ( B ) Cell viability assessed with fixable EF 780 viability dye back gated in side scatter (SSC) and forward scatter (FSC)-contour plot with outliers as dots. Green: viable cells; red: dead cells. Proportion expressed as a mean ± SD, n = 3. ( C , D ) Quantitative polymerase chain reaction assessing DCN (coding for Decorin) ( C ) and CLEC4F (coding for Clefc4f) ( D ) gene expression in cell populations after the two major steps of HSC isolation (Nycodenz and MACS sorting). Values obtained were expressed as fold-increase (FI) where the value obtained by non-parenchymal cell (NPC) population was set as 1. Decorin has been described to be specific to HSCs whereas CLEC4F is expressed by Kupffer cells. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.00, if not specified, non-significant. n = 2.

Article Snippet: Nycodenz density gradient solution was from Axon lab AG (Le Mont-sur-Lausanne, Switzerland).

Techniques: Real-time Polymerase Chain Reaction, Gene Expression, Isolation

Journal: Cells

Article Title: Optimized Isolation and Characterization of C57BL/6 Mouse Hepatic Stellate Cells

doi: 10.3390/cells11091379

Figure Lengend Snippet: Cell yield per mouse after HSC Nycodenz separation alone or after additional negative CD11b selection. Nycodenz: yield without CD11b negative selection. CD11b−: HSC enriched fraction (flow through). CD11b+: macrophages (selected cells). SEM: standard error of the mean. Yield variations reflected fluctuations in liver digestion and learning curve of the isolation procedure. n = 3 to 7.

Article Snippet: Nycodenz density gradient solution was from Axon lab AG (Le Mont-sur-Lausanne, Switzerland).

Techniques: Selection, Isolation